Integrating social media and environmental DNA records to enhance surveillance and improve early detection of invasive species

A peer-reviewed open-access journal

@) NeoBiota

Advancing research on alien species and biological invasions

NeoBiota 102: 209-226 (2025)
DOI: 10.3897/neobiota.102.151710

Research Article

Integrating social media and environmental DNA records
to enhance surveillance and improve early detection of

invasive species

Diogo Dias'”®, Sofia Batista’, Sofia Nogueira’™®, Manuel Curto?*®, Diogo Ribeiro’, Rui Rivaes™,

Filipe Ribeiro’®

1 MARE, Marine and Environmental Sciences Centre / ARNET, Aquatic Research Network, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal
2 CE3C-cE3c - Centre for Ecology, Evolution and Environmental Changes/Global Change and Sustainability Institute, Faculty of Sciences, University of Lisbon,

1749-016 Lisbon, Portugal

3 CIBIO - Research Center in Biodiversity and Genetic Resources, InBIO Laboratorio Associado, Campus de Vairao, 4485-661 Vairao, Portugal
4 BIOPOLIS - Program in Genomics, Biodiversity and Land Planning, Campus de Vairao, 4485-661 Vairao, Portugal
Corresponding author: Diogo Dias (dmldias@ciencias.ulisboa.pt)

OPEN Qaceess

Academic editor: Paula Pappalardo
Received: 28 February 2025
Accepted: 9 July 2025

Published: 7 October 2025

Citation: Dias D, Batista S, Nogueira S,
Curto M, Ribeiro D, Rivaes R, Ribeiro

F (2025) Integrating social media

and environmental DNA records to
enhance surveillance and improve
early detection of invasive species.

In: Anastacio P Ribeiro F, Chainho P
(Eds) Invasions in Aquatic Systems.
NeoBiota 102: 209-226. https://doi.
org/10.3897/neobiota.102.151710

Copyright: © Diogo Dias et al.
This is an open access article distributed under
terms of the Creative Commons Attribution

License (Attribution 4.0 International - CC BY 4.0).

Abstract

The early detection of invasive species in aquatic ecosystems is particularly challenging with most
records of new species made years after their initial invasion, by which time they are already
widespread. Recently, early detection tools such as citizen science and environmental DNA have
emerged, significantly improving early warnings in aquatic ecosystems. However, there is limited
understanding of how these new tools complement each other and how consistent they are. In
this study, we present a case study combining social media data mining and environmental DNA
(eDNA) to detect new records of the invasive European perch (Perca fluviatilis) in mainland Portu-
gal. From 2021 to 2024, we analyzed online angling groups to identify potential new areas of inva-
sion. Later, water samples were collected from several reservoirs and tested for the European perch
presence using real-time quantitative PCR. This combined approach detected four new locations
of European perch. Moreover, eDNA analysis revealed three new potential populations, while
data mining appears to offer near real-time tracking of the species’ spread. This work showcases
the improved efficiency and early detection benefits of this integrated approach for monitoring

freshwater invasive fish, with broader applicability to other invasive species.

Key words: Data mining, detection lag, European Perch, freshwaters, non-native species, Perca

fluviatilis, GPCR

Introduction

Biological invasions are a global threat to biodiversity and ecosystem integrity, with
adverse economic and societal impacts (Lawler et al. 2006; Lodge et al. 2006; Eh-
renfeld 2010; Bradshaw et al. 2016). Furthermore, biological invasions and their
impacts are expected to continue to rise on par with current trends of global trade,
travel and transport (IPBES 2023). The ideal approach to mitigate the impact of
invasive species is to prevent their introduction into new areas (Lodge et al. 2006;
Finnoff et al. 2007). Following the introduction of invasive species, there is only

209

Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

a brief window during which eradication or control measures are both effective
and cost-efficient (Martinez et al. 2020). These actions are highly dependent on
early detection of invasive species, which plays a critical role in enabling fast re-
sponse measures, and is identified as a top priority in invasive species management
programs (e.g., Vander Zanden et al. 2010; IPBES 2023). Early detection con-
stitutes a challenge particularly in aquatic ecosystems, where new introductions
remain hidden beneath the surface, often going undetected for extended periods of
time and, consequently, enabling their establishment and spread (McDonald and
Thompson 2004; Jerde et al. 2011).

Traditional detection methods, relying on the physical capture of specimens, are
often labor-intensive, time-consuming and expensive, leading to a lack of broad geo-
graphical and temporal coverage (Amano et al. 2016). Consequently, there is a grow-
ing demand for innovative surveying approaches that address these weaknesses while
also enabling the early detection of invasive species in freshwater ecosystems (Dar-
ling and Mahon 2011). A recent new tool is based on the recovery of environmen-
tal DNA (eDNA), i.e., the material that organisms release into their surroundings
through processes such as shedding skin cells, secreting mucus, or releasing gametes
(Taberlet et al. 2012; Pawlowski et al. 2020). These methods are highly sensitive,
making them especially suited for detecting early signs of invasive species (Carim et
al. 2019; Duarte et al. 2023) or assessing the success of eradication actions (Davison
et al. 2019). Additionally, their efficiency and cost-effectiveness greatly enhance the
viability of large-scale monitoring programs (Coble et al. 2019). These approaches
can target specific taxa using methods like qPCR or use high-throughput sequencing
to characterize biological communities (Taberlet et al. 2012).

The use of eDNA in early detection of invasive species may be particularly advanta-
geous when integrated with other techniques such as social media data mining, which
can identify areas of potential concern and guide targeted eDNA sampling efforts (Par-
rondo et al. 2018). In the digital era, a rising number of recreational anglers turn to
internet forums and social networks to share their experiences and connect with people
with similar interests (Giovos et al. 2018). Individuals often upload text, photographs
and videos relating to their fishing activity to social media, contents that are valuable
for fisheries sciences (Vitale et al. 2021; Lennox et al. 2022). Given the key role of an-
glers in the spread of invasive fish species, particularly in the Iberian Peninsula (Ribeiro
et al. 2009; Banha and Anastacio 2015; Banha et al. 2024), this growing source of
information may represent a cost-effective source of new records that can be used to
track the spatial occurrence of new invasive fishes (Gago et al. 2016; Banha et al. 2017).
These data have the potential, not only to complement already existing invasive spe-
cies monitoring programs and methodologies with few added resources and expenses
(Daume 2016; Gago et al. 2016; Allain 2019), but also to work as a sentinel tool for a
prompt notice of new introductions, and effort redirection.

In Iberia, freshwater fish are among the most threatened taxonomic group (Cos-
ta et al. 2021), with invasive species widely recognized as a major threat (Hermo-
so and Clavero 2011; Zamora-Marin et al. 2023). However, most recent Iberian
studies on freshwater fish invasions have primarily focused on three aspects: re-
porting first occurrences (Ribeiro and Verissimo 2014; Banha et al. 2015), as-
sessing their impacts on native fauna (Ribeiro et al. 2021; Gkenas et al. 2022), or
mapping their distribution long after establishment (Banha et al. 2017; Martelo
et al. 2021). In fact, there is still limited information about the range expansion
of recently arrived invasive fish (but see Gago et al. 2016), making it difficult to

NeoBiota 102: 209-226 (2025), DOI: 10.3897/neobiota.102.151710 210

Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

identify secondary introductions by anglers within specific regions, limiting effec-
tive actions to prevent further spread (Caffrey et al. 2014).

Portuguese freshwaters have been highly invaded by different taxa (Anastacio et
al. 2019; Zamora-Marin et al. 2023), with one new fish species introduced approx-
imately every two years over the past two decades (Ribeiro et al. 2009; Banha et
al. 2017). Of these, one of the most recently introduced predators is the European
perch (Perca fluviatilis Linnaeus, 1758). This species was introduced in 1898 in the
Azores Archipelago by the Forestry Services to promote angling activities (Goubier
et al. 1983; Ribeiro et al. 2009). However, it was first detected on the mainland
in 2013 (Banha et al. 2015). This species is native to parts of Asia and Europe but
naturally absent in southern European Peninsulas (Stepien et al. 2015). Due to its
recreational popularity, it has been introduced in many other regions of Europe
(Italy, Spain and Cyprus), Africa (Morocco and South Africa), Asia (China) and
Oceania (Australia and New Zealand) (Stepien et al. 2015; Ning et al. 2025) with
documented negative impacts on these aquatic ecosystems (Morgan et al. 2002;
Morgan et al. 2004). This study aimed to assess whether social media records of
European perch could help guide an eDNA-based monitoring campaign targeting
this invasive fish and monitor its range expansion across mainland Portugal. Using
a recently introduced fish in Portugal as a case study, we sought to show the po-
tential of integrating species records from social media data mining, eDNA-based
monitoring, and scientific fishing to evaluate the complementarity between these
approaches and identify invasion routes and secondary introduction locations.

Methods
Study area

In 2013, the European perch was confirmed on mainland Portugal for the first
time, in a small reservoir in Proenga-a-Nova (Industrial Park of Proen¢a-a-Nova -
PEPA, 39°43'19.9"N, 7°53'58.6"W), located in the central region of the country
(Banha et al. 2015). This remained the only known introduction site until 2021,
when a new population was recorded in a large reservoir in Sabugal (40°19'57.8"N,

7°05'27.9" W), situated in a different drainage basin (Fig. 1A).

Data mining

We collected information on anglers’ catches of European perch from publicly available
social media posts on Facebook™, a popular platform among anglers in Portugal with
the largest freshwater angling group hosting over 30,000 members, and YouTube™.
On both social media websites, potentially relevant posts were identified by
using the search tool with selected key-words. Search keywords included the spe-
cies common name in Portuguese (“perca europeia’) and variants, such as “perca’,
“percilha” and “perche”. Additionally on Facebook, searches within fishing-groups,
such as “Pesca em Agua Doce” and “Big Perch Monster” were conducted to iden-
tify posts related to the target species, as these groups harbor large and engaged
communities on Portuguese and perch angling, respectively. The dataset included
posts since May 2021, when the first record of European perch appeared on Face-
book™, until December 2024. All collected posts were manually reviewed to con-
firm species identification, capture location and date, while the raw data was used

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Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

to analyze publication trends through time. Then, only posts explicitly related to
recreational fishing for European perch, i.e., videos or photographs of catches of
perch were selected for further analysis. For each post, we extracted information
on the catch’s date and location, and compiled data from the different sources into
one final dataset. The data collection process adhered to ethical guidelines for so-
cial media use in fisheries research, ensuring no personal information was recorded
and no original user content was shared publicly (Monkman et al. 2018).

Fish sampling

To confirm the presence of suspected European perch populations reported on
social media, field sampling was conducted in suspected locations in February
2023. Gillnets following the European standard EN 14 757 (CEN 2005) were
deployed and left overnight. Subsequent to these results, nearby streams were
sampled using electrofishing equipment (Hans Grass! EL 6v2 generator, DC,
600 V) to evaluate potential perch dispersal beyond the reservoirs. Electrofishing
was conducted along a 120-meter stretch of the stream using a zigzag wading
pattern. All captured fish’ total length was measured. Native species were re-
leased, while invasive species were euthanized in compliance with local regula-
tions using a clove oil overdose as an anesthetic. The presence of European perch
was confirmed by the capture of at least one individual.

Environmental DNA samples collection

Site selection for eDNA analysis was informed by a combination of social media
data mining and species distribution records (Fig. 1A, B). Sampling was con-
ducted on reservoirs with known populations of European perch as positive con-
trols, such as Sabugal and PEPA. Nearby and hydrologically connected reservoirs
were sampled to prospect the existence of undetected populations. The sampling
effort was determined based on water body size: one filtration site in small reser-
voirs (<15 ha), two in medium (15-150 ha) and five in large reservoirs (>150 ha)
or rivers. Sites within each reservoir were selected to be as widely dispersed as
possible to maximize detection probability.

Between the 24" of June and the 24" of July of 2023, eDNA samples were
collected at 52 sites across the hydrographic basins of the Tagus (32 sites), Dou-
ro (13 sites), and Ave (7 sites) (Fig. 1C). At each site, we filtered 30 | of water
through a 0.45 um pore-size filter (GoPro™ High Capacity Groundwater Filter,
Proactive, Florida, USA) connected by a plastic hose to a diaphragm water pump
(Argaly, Sainte-Helene Du Lac, France) with a flowmeter attached to the pump
outlet to monitor the filtered volume. Our target filtration volume (30 |) was set
to maximize species detection, as recommended by Cantera et al. (2019). The
filtration process was stopped early in the case of filter clogging, which we de-
fined as when the flow rate dropped below 1 | per 5 min. Upon completing the
filtration, we loaded 50 ml of preservation buffer (Longmire et al. 1997) into the
filter capsule and kept samples at 4 °C until laboratory analysis.

Field campaigns adhered to strict contamination control measures: the hose
connecting the filter capsule to the inlet of the pump was decontaminated prior
to use and discarded afterwards; the syringe used for loading the buffer was sin-
gle-use; each filter capsule was capped, labeled and sealed individually inside a

NeoBiota 102: 209-226 (2025), DOI: 10.3897/neobiota.102.151710 212

Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

Bouga-Cova
@

®
=v Data mining

@ Detections

Populations
OD) eDNA ‘ > ‘ @ Confirmed
o © Negati Y \
egative \
© Inconclusive
O Positive

Penha Garcia

Mo ® ; j
es o
on ot

a

Figure 1. Study area maps of European perch distribution and detections in Portugal: A. Known European perch (Perca fluviatilis) distri-
bution in Portugal and its relative position in Europe; B. Results of social media data mining, where occurrences of European perch were
identified through online sources; C. Environmental DNA sampling grid and results, where in white are negative results, yellow — incon-

clusive detections and in purple positive detections; D. Confirmed European perch populations through all methods.

decontaminated zipped plastic bag; waders used during sampling were decontam-
inated between sites; gloves were worn at all times and exchanged between sites or
as needed within the same site. Decontamination consisted of soaking the material
in a solution of 20% (v/v) commercial bleach for at least 30 min (40% bleach for
boots). Field blanks were processed by filtering 6 | of bottled water at the first site
each day (one blank per day), following the same protocol as regular samples. A
rental car was used, instead of a research institute car, to avoid contamination from
previous field trips. Populations identified through social media data mining pos-
teriorly to the eDNA campaign were not sampled for eDNA.

Development of a real-time PCR assay specific for Perca fluviatilis

New primers and probe for the European perch were designed due to the absence of
published primers at the time that could provide sufficient specificity for our target
species in the study area. The detailed procedure for designing and validating the new
primers and probe is provided in Suppl. material 1. Briefly, we compiled sequences for
two mitochondrial genes for the Percidae family from National Center for Biotech-
nology Information (NCBI) nucleotide database (https://www.ncbi.nlm.nih.gov)

NeoBiota 102: 209-226 (2025), DOI: 10.3897/neobiota.102.151710 213

Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

and aligned each database using MAFFT (Katoh et al. 2019). PrimerQuest™ was
used to identify primer and probe candidate regions in the target species and these
regions were subsequently manually refined based on the reference alignments visu-
alized in BioEdit (Hall 1999). Regions were selected with the intent of maximizing
mismatches with non-target species, particularly Sander lucioperca (the only other
percid in the study area), while ensuring no mismatches with the target species.
Candidate sets were assessed with OligoAnalyzer™ for thermodynamic properties
(Im, GC content, secondary structures). Specificity was evaluated in silico using
eDNAssay (Kronenberger et al. 2022) against a comprehensive sequence database of
23 fish species from the study area (also retrieved from NCBI’s database). EDNAssay
is a freely available web-based tool (https://nationalgenomicscenter.shinyapps.io/
eDNAssay) that uses machine learning (random forest models) to predict the likeli-
hood of amplification between a set of primers/probe and a set of DNA sequences.
Among all sets tested, the PfluCytb primer-probe set showed the highest amplifica-
tion probability for P fluviatilis with a very low likelihood of cross-amplification in
non-target species. This set of primers (PfluCytb-F: 5’-CCTCCATCCTGGTTCT-
TATAGTT-3’; and PfluCytb-R: 5’°-AGGATAACAACATCTGCGATTAATGT-3’)
and probe (PAluCytb-P: 5°-ATTGGGAGAGCGGTCGGAATGTAATGCCA-3’)
targets a 113 base pair region of the Cytochrome b mitochondrial gene. Jn vitro
validation was performed on tissue samples from the target species and four sympat-
ric non-target species, including Sander lucioperca and other locally abundant taxa.
At this stage, no additional percid species were tested beyond those present at our
study site, which should be considered when applying this assay in other regions.
The qPCR assay was performed using the QuantStudio™ 5 Real-Time PCR System
(Thermo Scientific, Massachusetts, USA) under the following protocol: 10 s at 95
°C, followed by 40 cycles of 15 s at 95 °C and 60 s at 60 °C. Each PCR reaction con-
tained a total volume of 10 ul, consisting of 5 ul TaqMan™ Environmental Master
Mix 2.0 (Applied Biosystems, California, USA), 0.4 ul each primer (both at 10 uM;
Stab Vida, Caparica, Portugal), 0.13 ul of probe (100 uM; Eurofins, Luxembourg),
3.07 ul of sterile ultrapure water, and 1.0 pl of DNA. Reactions were run in dupli-
cate and negative controls included. The PfluCytb set showed clear amplification
only in the target species, confirming its specificity.

Assay performance was evaluated using a 5-fold serial dilution of target species’
DNA, following the same protocol as above except for the DNA volume (2.0 pl)
and water volume (2.07 ul) and all concentrations were run in triplicate. The stan-
dard curve demonstrated high linearity (R’ = 0.9973) and efficiency (101.3%),

with Ct variability < 0.3 across replicates.

Laboratory tests of environmental samples

Laboratory processing of the eDNA samples, including DNA extraction and PCR,
was conducted in dedicated facilities exclusively used for handling non-invasive
samples, meaning material collected without capturing, handling, or directly in-
teracting with the target organism. DNA extractions were performed using the
DNeasy Blood and Tissue Kit (Qiagen, Inc., Hilden, Germany), following the
manufacturers guidelines with few modifications detailed in Suppl. material 2.
In each extraction batch, 6 randomly chosen samples were processed alongside an
extraction blank (from initial 50 ml of Longmire buffer). Extraction success was
assessed by agarose gel electrophoresis (0.8%).

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Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

All eDNA samples and blanks were tested in triplicate for the presence of Euro-
pean perch using the last protocol described above. Each qPCR run included pos-
itive controls (genomic DNA European perch at 78.97 ng/ul) and a negative con-
trol (sterile ultrapure water). A replicate was considered positive if an exponential
amplification curve was observed (cycle threshold, C, < 34). In cases where some
amplification was observed but the amplification curve did not follow a general
exponential pattern), the replicate was considered inconclusive. Sites were deemed
positive if at least two replicates displayed C,, values below 34, and negative if none
of the replicates showed positive amplification. In cases where only one replicate
was positive, or if there were inconclusive results across replicates (e.g., one posi-
tive, one inconclusive, and one negative), we considered the presence of European
perch in the sample as “Inconclusive”.

Given that eDNA detectability can be affected by several environmental factors
which can lead to false negatives, we ran two additional qPCR assays, with adjust-
ments in sample input volume. In one essay, we halved the input volume of the
sample (1 pl) to reduce the impact of potential inhibitors, and, in the other assay,
we used an input sample volume two times higher than in the original assay (4 ul)
to account for very low concentrations of eDNA at the sampling sites (Nogueira et
al. 2025). These two additional assays were run for all the samples with negative or
inconclusive results in the original assays and followed the same protocol as above.

A reservoir/river was classified as positive or inconclusive if at least one site with-
in it yielded a positive or inconclusive result, following a precautionary principle.

Results

A total of 153 social media posts related to European perch were identified between
May 2021 and December 2024 (Fig. 2; See Suppl. material 3). Of these, 43 posts
were excluded from the species distribution analysis due to the absence of photo-
graphic evidence, lack of capture-related content, or references to locations outside
mainland Portugal (e.g., the Azores, France, and Spain). These excluded posts typ-
ically included fishing-related questions or general information about the species.
The remaining 110 posts (100 from Facebook and 10 from YouTube) were analyzed
to determine capture locations based on location tags, post descriptions, comments,
and photographic records. In addition to known populations in PEPA and Sabugal,
two new locations, Batocas and Meimoa (Figs 1B, 2), were identified in multiple
posts between 2021 and 2023. A new reservoir, Bouca Cova, was referenced in posts
beginning in July 2024 (Fig. 2). The number of social media posts has increased ex-
ponentially over time, with the arrival of the species in new locations triggering a pro-
portional rise in publications. Moreover, the invaded area also increased dramatically
with the second location, which is the largest reservoir in surface area and currently
this invasive fish has occupied a total of 1000 ha in Portuguese watersheds (Fig. 3).
Fish sampling confirmed the presence of European perch in both Batocas and
Meimoa reservoirs. A total of 57 individuals were captured in Batocas and 27 in
Meimoa, with sizes ranging from 7.9 cm to 23.7 cm, predominantly comprised
by mature individuals. Electrofishing conducted upstream and downstream of the
four known population sites (at the time) captured ten European perch upstream
of Meimoa reservoir and one individual downstream of the dam. No European
perch were detected in streams outside the reservoirs associated with the other

three sampled populations (PEPA, Sabugal, and Batocas).

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Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

Boga Cova

e New Occurrence

— Social Media Posts
Meimoa

Cumulative posts (count)

Batocas
PEPA Sabugal
2015 2017 2019 2021 2023 2025
Date

Figure 2. Cumulative social media mentions of European perch: Cumulative number of social me-
dia posts detected regarding the European perch through time, with the first occurrence of each

population marked in red.

El eDNA
_ Social Media
Suspected Invaded Area

Cumulative Invaded Area (ha)

2015 2017 2019 2021 2023 2025
Date

Figure 3. Cumulative invaded area by European perch in Portugal: Cumulative invaded area by Eu-

ropean perch through time, with social media assessed detections in green, cDNA positive detections

in purple and eDNA inconclusive detections in Yellow.

Under the original qPCR conditions, European perch was detected in 11 of the 52
sampled sites (Fig. 1C, Table 1). In all sites with positive eDNA detections, all three
qPCR replicates were positive. All eight sites sampled within the four reservoirs con-
firmed to host European perch populations tested positive: PEPA (one site), Sabugal
(three sites), Meimoa (three sites) and Batocas (one site). The rivers downstream of
these reservoirs tested positive in Meimoa and Sabugal, whereas in PEPA and Bato-
cas tested negative. The remaining positive detection represented a new location for
this species in Penha Garcia reservoir (Fig. 1C). This occurred at one of the two sites
sampled in the reservoir, with the other site at this reservoir yielding an inconclu-
sive result (one positive out of three replicates). Two other reservoirs, Marateca and
Pracana, where five sites were sampled within each reservoir, as well as the Ave River,
where five sites were sampled along the river, yielded one inconclusive site each for
the presence of European perch (Fig. 1C). The additional tests varying sample input
resulted in inconclusive detections for one site in Penha Garcia under diluted con-
centration conditions, and one in Pracana under increased concentration conditions.
No European perch DNA was detected in the remaining sites across 11 reservoirs
(Fig. 1C). None of the negative controls added showed signal of amplification.

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Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

Table 1. Summary of main results by location, including main location coordinates (primarily reservoirs; lotic systems are indicated

with *) that are representative of the sampled water body, and the number of filtration sites per location (see Suppl. material 4 for details).

European perch detection is reported by method: “X” indicates detection (even if inconclusive), “O” indicates no detection, and “N/A”

denotes that the method was not applied at that site. The table also identifies the method by which the species was first detected at each

location and evaluates the likelihood of an established European perch population based on the detection evidence.

Main locations
(reservoirs/rivers)

Penha-Garcia
Idanha-a-Nova
Marateca
Pracana

PEPA

Pisco

P. Redondo
Capinha

Monte Bispo
Escarigo
Meimoa
Meimoa stream*
Batocas

Sabugal

Céa River*
Vascoveiro

Sra do Monforte
Vermiosa

Sta Maria do Aguiar
Ermal

Ave River*

Bouca Cova

Coordinates

40°02'44.3"N, 7°00'54.5"W
39°56'41.1"N, 7°12'00.0"W
39°58'10.2"N, 7°28'57.0"W
39°33'54.1"N, 7°48'43.5"W
39°43'21.0"N, 7°53'57.6"W
40°01'24.8"N, 7°33'23.6"W
40°03'44.8"N, 7°32'24.8"W
40°12'53.3"N, 7°22'59.1"W
40°15'30.9"N, 7°19'23.2"W
40°15'25.9"N, 7°17'10.1"W
40°15'33.7"N, 7°08'23.7"W
40°15'33.7"N, 7°08'23.7"W
40°28'49.9"N, 6°51'18.6"W
40°19'53.2"N, 7°05'42.5"W
40°19'53.2"N, 7°05'42.5"W
40°42'42.2"N, 7°05'39.0"W
40°47'12.7"N, 7°00'43.1"W
40°48'17.7"N, 6°53'20.0"W
40°51'52.7"N, 6°53'09.4"W
41°35'05.4"N, 8°08'10.1"W
41°21'17.2"N, 8°27'21.7"W
40°41'59.3"N, 7°14'48.2"W

Filtration sites | Social media | eDNA Selcnaue 1* Suspicion Presence
fishing

2 0 4 N/A eDNA Highly likely
. 0 0 N/A — No evidence
5 0 Xx 0 eDNA Somewhat likely
5 0 Xx 0 eDNA Somewhat likely
2 xX Xx xX Social Media Confirmed
2 0 0 N/A - No evidence
1 0 0 N/A - No evidence
il 0 0 N/A - No evidence
1 0 0 N/A - No evidence
d 0 0 N/A — No evidence
3 xX xX XxX Social Media Confirmed
1 0 X X Scientific Fishing Confirmed
2 xX Ms xX Social Media Confirmed
3 xX xX xX Social Media Confirmed
1 0 x N/A eDNA Highly likely
2 0 0 N/A - No evidence
1 0 0 N/A - No evidence
2, 0 0 N/A - No evidence
2 0 0 N/A - No evidence
2 0 0 N/A - No evidence
3 0 Xx N/A eDNA Highly likely
0 x N/A N/A Social Media Highly likely

Discussion

In this study, we show that social media data mining of invasive fish can effectively
guide targeted eDNA-based monitoring, optimizing the search around known or
suspected locations and, consequently, confirm recently established populations.
By analyzing nearby locations likely to be colonized, as well as potential fishing
hotspots, we identified a total of four unreported populations and three suspected
localities of European perch in mainland Portugal (Fig. 1C, D, Table 1). Prior to
this work, European perch was known to be present in only two reservoirs (PEPA
and Sabugal, Fig. 1A), occupying an area of 714 ha which has currently risen to
1026 ha. This approach significantly reduced the species detection time lag, be-
tween its arrival and confirmation in new areas, enabling more timely and effective
management responses, such as local containment or local eradication.

The rise of social media has facilitated anglers’ sharing of fishing experiences
online, creating a valuable and publicly accessible data source (Lennox et al. 2022).
By monitoring European perch related publications, we identified previously un-
known populations in three reservoirs, Batocas, Meimoa and Bouga Cova (Fig. 1B,
Table1). ‘The potential of this approach for monitoring this species was first demon-
strated in its first record in mainland Portugal (Banha et al. 2015), which relied
on data from online fishing forums. Other studies further demonstrated this with

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Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

regards to other species, such as the European catfish (Parrondo et al. 2018; Gkenas
et al. 2023) and roach (Ribeiro and Verissimo 2014). Since its initial detection,
only one European perch population had been reported through a fisherman’s re-
port to the national environmental agency (ICNF) in 2021. Although not analyzed
in this study, social media also offers insights into anglers’ perceptions of invasive
species and management actions and the respective sentiment analysis could guide
communication strategies, improve stakeholder engagement, and help foster public
acceptance of management initiatives (Lennox et al. 2022; Sbragaglia et al. 2022).
However, social media data mining also has limitations (Ghermandi and Sinclair
2019; Jari¢ et al. 2020). Posts can lack reliability, and the need for cross-verifica-
tion remains crucial (Jarié et al. 2020; Lennox et al. 2022). In our analysis, we
observed instances of species misidentifications, with common examples including
the confusion between European perch and other species such as pikeperch (Sander
lucioperca) or pumpkinseed perch (Lepomis gibbosus), a recurring issue in Portugal
(Venturelli et al. 2017). Additionally, many members of the public, particularly
within angling communities, still believe that natural biotic communities can be
improved and thus do not fully recognize the need to protect biodiversity (Cam-
bray 2003). Distrust of scientific institutions within some fishing communities has
also led to the withholding of critical information, such as capture locations, from
some anglers in Portugal (Dias D., personal observation). Data availability can also
be biased towards popular fishing spots and larger reservoirs, limiting insights into
less visited, but ecologically significant areas. This bias is evident in Fig. 2, where
publication frequency increased significantly after the discovery of populations in
larger reservoirs like Sabugal and Meimoa in comparison to the number of publi-
cations regarding smaller reservoirs and more remote sites such as Batocas. Other
biases have been reported in the literature such as socio-economic, ethical and de-
mographic (Ghermandi and Sinclair 2019; Jari¢ et al. 2020), and remain one of
the challenges iEcology, “the study of ecological patterns and processes using online
data generated for other purposes and stored digitally” (Jari¢ et al. 2020), still faces.

In this study, EDNA complemented social media data mining. Water was sam-
pled from reservoirs less visited by anglers, addressing the bias previously reported
for larger and more popular fishing spots. This resulted in a strong indication of a
possible new population in Penha Garcia reservoir (Fig. 1D, Table 1), a site not yet
mentioned on social media, underscoring eDNA’s potential to uncover species in
remote or poorly studied areas (Goldberg et al. 2016). In two other reservoirs and
one river, inconclusive eDNA results suggested the possible presence of European
perch populations. Although no specimens have been recovered from these two res-
ervoirs, this does not necessarily contradict the eDNA findings. DNA-based meth-
ods are significantly more sensitive than traditional fishing techniques (Goldberg et
al. 2016), enabling species detection even in the absence of physical captures. Fur-
thermore, eDNA sampling facilitated the survey of multiple locations within a short
timeframe, offering a cost-effective and high-throughput alternative to traditional
methods, such as direct observation or captured-based surveys (Loeza-Quintana et
al. 2020). By targeting less-accessible reservoirs and early stages of invasion, eDNA
provided critical insights that could guide early intervention and management strat-
egies (Harrison et al. 2019). In aquatic environments, DNA sampled at a river site
is the result of local DNA traces and genetic material transported from upstream
sources along the river network (Carraro et al. 2020). Therefore, the eDNA detec-
tion in downstream reservoirs — Meimoa and Sabugal - may not correlate with local

NeoBiota 102: 209-226 (2025), DOI: 10.3897/neobiota.102.151710 218

Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

species presence and should thus warrant regular monitoring to access species dis-
persal downstream of invaded reservoirs. One potential new population identified
through social media (Bouca Cova) was not included in the eDNA sampling because
it had not been referenced at the time of the survey campaigns.

Several factors can influence species detection using eDNA (Goldberg et al.
2016; Loeza-Quintana et al. 2020; Burian et al. 2021). To address possible false
negatives, we applied a dual strategy in the qPCR assay: testing samples at both
diluted and concentrated final volumes. This approach led to inconclusive results
for European perch in two additional reservoirs (Fig. 1C). There was no evidence
of contamination in the associated blanks of these sites. The lack of reproducibility
in these samples may also reflect low concentration of target eDNA due to low
species abundance in these systems or heterogeneous dispersal patterns of both the
organism and eDNA itself (Pont et al. 2018; Buxton et al. 2021). At one of these
sites in the Ave River, where the presence of European perch is not officially rec-
ognized, an independent study detected the presence of the European perch using
eDNA metabarcoding and metagenomics from a water sample collected in 2020
(Curto et al. 2025). Taken together, these findings constitute stronger evidence
that the Ave drainage is worthy of further monitoring and support the possibility
that in many of the inconclusive sites the European perch might be indeed present.
As the use of eDNA in invasive species management will continue to grow (Abbott
et al. 2021), clear guidelines are still needed to define how conservation managers
should respond to eDNA detections. Given that capture-based surveys remain the
gold standard for confirming species’ presence, there is a need to allocate adequate
resources to support follow-up efforts when eDNA signals raise concerns.

The European perch is in the early stages of invasion in Portugal. Following the
first confirmed record (Banha et al. 2015), there was an eight-year gap before a second
population was identified. However, the rate of new population discoveries has acceler-
ated, with four new populations confirmed in the past 18 months and three additional
suspected. ‘This rapid spread of an invasive species underscores the urgency for man-
agement efforts to control dispersal and mitigate impacts on newly invaded systems
(Strayer et al. 2017). Monitoring programs are essential to address the rapid changes in
fish communities and to track the distribution of invasive species in freshwater systems
(Epanchin-Niell 2017). This study also reports the first confirmed occurrence of Eu-
ropean perch in a lotic system in Portugal (Meimoa stream), revealing its ability to by-
pass dams and colonize downstream habitats. These habitats are particularly vulnerable
(Hermoso and Clavero 2011), as they often harbor Iberian endemics lacking natural
defenses against novel predators like the European perch (Leunda 2010).

In conclusion, this study demonstrates the strong potential of integrating social
media data mining with environmental DNA (eDNA) methods for proactive inva-
sive species management. By expanding the monitoring framework to include both
the confirmation of suspected populations and the discovery of previously undoc-
umented ones, we significantly enhanced the detection of European perch. The
combined use of social media and eDNA has the potential to be a cost-effective and
timely approach to detecting invasive species in newly affected areas, even uncov-
ering locations not initially perceived as spatially connected to known populations,
thereby bridging the gap between species introduction and effective management
(Fig. 1D, Table 1). This approach not only improves early detection of invasive spe-
cies but also enables more responsive, targeted, and resource-efhicient conservation
actions, ensuring that management efforts are both informed and timely.

NeoBiota 102: 209-226 (2025), DOI: 10.3897/neobiota.102.151710 219

Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

Acknowledgements

The authors are also thankful for the assistance of volunteers in the field campaigns,

namely Miguel Rodrigues, Gil Santos and Daniel Mendes.

Additional information

Conflict of interest

The authors have declared that no competing interests exist.

Ethical statement

No ethical statement was reported.

Use of Al

No use of AI was reported.

Funding

This study was conducted within the framework of the project MEGAPREDATOR (FCTiref. PTDC/
ASP-PES/4181/2021; https://doi.org/10.54499/PTDC/ASP-PES/4181/2021), co-funded by the
European Commission under the EU LIFE Nature & Biodiversity 422 Project programme (Project
101074458 — LIFE21-NAT-IT-PREDATOR). This publication was financed by the Fundagao para a
Ciéncia ea Tecnologia (FCT) through the project UID/04292-Centro de Ciéncias do Mar e do Ambiente,
awarded to MARE and through project LA/P/0069/2020 (https://doi.org/10.54499/LA/P/0069/2020)
granted to the Associate Laboratory ARNET. FCT funding awarded to the institution “Centro de
Ecologia, Evolugao e Alteragoes Ambientais” with reference UIDB/00329/2020, DOI 10.54499/
UIDB/00329/2020 (https://doi.org/10.54499/UIDB/00329/2020). D. Dias (2023.01409.BD, https://
doi.org/10.54499/2023.01409.BD) is supported by FCT with a PhD grant. C. Gkenas (DL57/2016/
CP1479/CT0036) and FE Ribeiro (CEEC/0482/2020, https://doi.org/10.54499/2020.00482.CEEC-
IND/CP1595/CT0001) are supported by individuals’ contracts from FCT.

Author contributions

Dias, D. — Conceptualization, Writing - original draft, Writing - review and editing, Data curation,
Investigation, Methodology; Batista S. — Writing - original draft, Writing - review and editing, Data
curation, Formal analysis, Investigation; Nogueira S. — Writing - original draft, Writing - review
and editing, Data curation, Formal analysis, Investigation, Methodology; Curto M. — Conceptual-
ization, Writing - review and editing, Investigation, Supervision; Ribeiro D. — Writing - review and
editing, Investigation, Methodology; Rivaes R. — Writing - review and editing, Funding acquisition,
Investigation; Ribeiro F— Conceptualization, Writing - original draft, Writing - review and editing,

Funding acquisition, Investigation, Project administration, Supervision

Author ORCIDs

Diogo Dias © https://orcid.org/0000-0003-2638-8923
Sofia Batista © https://orcid.org/0000-0003-3802-510X
Sofia Nogueira © https://orcid.org/0000-0001-6654-5300
Manuel Curto ® https://orcid.org/0000-0002-1630-4653
Diogo Ribeiro © https://orcid.org/0000-0002-1820-3557
Rui Rivaes © https://orcid.org/0000-0001-79 10-4387
Filipe Ribeiro ® https://orcid.org/0000-0003-3531-5072

NeoBiota 102: 209-226 (2025), DOI: 10.3897/neobiota.102.151710 220

Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

Data availability

All of the data that support the findings of this study are available in the main text or Supplementary

Information.

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Supplementary material 1

DNA extraction of environmental samples collection

Authors: Sofia Batista, Sofia Nogueira

Data type: docx

Copyright notice: This dataset is made available under the Open Database License (http://opendata-
commons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement
intended to allow users to freely share, modify, and use this Dataset while maintaining this same

freedom for others, provided that the original source and author(s) are credited.

Link: https://doi.org/10.3897/neobiota.102.151710.suppl1

NeoBiota 102: 209-226 (2025), DOI: 10.3897/neobiota.102.151710 225

Diogo Dias et al.: Combining social media and eDNA to enhance invasive species early detection

Supplementary material 2

Development of a real-time PCR assay specific for Perca fluviatilis

Authors: Sofia Batista, Sofia Nogueira

Data type: docx

Copyright notice: This dataset is made available under the Open Database License (http://opendata-
commons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement
intended to allow users to freely share, modify, and use this Dataset while maintaining this same

freedom for others, provided that the original source and author(s) are credited.

Link: https://doi.org/10.3897/neobiota. 102.151710.suppl2

Supplementary material 3

Data mined information from social media

Authors: Diogo Dias

Data type: xlsx

Copyright notice: This dataset is made available under the Open Database License (http://opendata-
commons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement
intended to allow users to freely share, modify, and use this Dataset while maintaining this same

freedom for others, provided that the original source and author(s) are credited.

Link: https://doi.org/10.3897/neobiota. 102.151710.suppl3

Supplementary material 4

Summary table of results

Authors: Diogo Dias

Data type: docx

Copyright notice: This dataset is made available under the Open Database License (http://opendata-
commons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement
intended to allow users to freely share, modify, and use this Dataset while maintaining this same

freedom for others, provided that the original source and author(s) are credited.

Link: https://doi.org/10.3897/neobiota. 102.151710.suppl4

NeoBiota 102: 209-226 (2025), DOI: 10.3897/neobiota.102.151710 226